| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177240 |
| Chromosome: | chromosome 7 |
| Location: | 1286768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321900 | (1 of 6) 3.1.26.11 - Ribonuclease Z / tRNase Z | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGGAAATTGTTGTACTGGGGACTGGT |
| Internal bar code: | GAGGATCCTGTGTGAGCTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 214 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTGTAATCAGCGCACAG |
| Suggested primer 2: | ACTGAAATGGTGTTCCCTGC |