Insertion junction: LMJ.RY0402.177253_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.177253
Chromosome:chromosome_7
Location:5180697
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGTGACGACCACCACCTCGACTTCTACTT
Internal bar code:GGTGGAGAGCGAGCTGGAGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:51
LEAP-Seq percent confirming:88.5714
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCGAACAGTCGGAAGCTAAG
Suggested primer 2:GTAGGCAATTCGGAACGTGT