Insertion junction: LMJ.RY0402.177253_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.177253
Chromosome:chromosome_7
Location:5180819
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g348450 antisense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTCAGTAAGACACTCAGAGCTCATACGTAG
Internal bar code:CCCCGTTACTTTGTGCCATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:123
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGCAATTCGGAACGTGT
Suggested primer 2:CCGAACAGTCGGAAGCTAAG