Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.177253 |
Chromosome: | chromosome_7 |
Location: | 5180819 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g348450 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCAGTAAGACACTCAGAGCTCATACGTAG |
Internal bar code: | CCCCGTTACTTTGTGCCATTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 123 |
LEAP-Seq percent confirming: | 93.75 |
LEAP-Seq n confirming: | 15 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTAGGCAATTCGGAACGTGT |
Suggested primer 2: | CCGAACAGTCGGAAGCTAAG |