Insertion junction: LMJ.RY0402.177267_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.177267
Chromosome:chromosome 6
Location:6791903
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g295001 (1 of 100) IPR001810 - F-box domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATACCCAGAAAGGAAGGGAAGGAAGATGG
Internal bar code:CTCACTCATCGGCGCCGAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:725
LEAP-Seq percent confirming:99.4966
LEAP-Seq n confirming:1779
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACGGGGTTGACTTTGAA
Suggested primer 2:AACGTCACCACACCAGTTGA