Insertion junction: LMJ.RY0402.177267_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.177267
Chromosome:chromosome 6
Location:6791904
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g295001 (1 of 100) IPR001810 - F-box domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGCTACCGTAACTCCCCCCCCGCCCCA
Internal bar code:TGGATCTAGTACAGACGATGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:102
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACGGGGTTGACTTTGAA
Suggested primer 2:AACGTCACCACACCAGTTGA