| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177282 |
| Chromosome: | chromosome 12 |
| Location: | 4020011 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517050 | FAP117 | (1 of 7) PF07004 - Sperm-tail PG-rich repeat (SHIPPO-rpt); Flagellar Associated Protein 117 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCTGCCGCGCTCCCCTGCATACCGTAT |
| Internal bar code: | GTACTCGACTGGTCATACGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 96.1598 |
| LEAP-Seq n confirming: | 626 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGATAGTGATGGGGAGC |
| Suggested primer 2: | GCGCAAGGTGTTTGTGTCTA |