| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177297 |
| Chromosome: | chromosome 14 |
| Location: | 1399385 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617450 | HSP22E | Heat shock protein 22E; (1 of 4) PTHR11527//PTHR11527:SF128 - SMALL HEAT-SHOCK PROTEIN HSP20 FAMILY // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCCGCGCCCTCAAATCCCGAGCGGGCT |
| Internal bar code: | CTCTAAACCATGTGGGCCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 99.8259 |
| LEAP-Seq n confirming: | 1147 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAAGTCAGTCGCTTCCA |
| Suggested primer 2: | TGTTACAGGTATGGCCGTGA |