| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177299 |
| Chromosome: | chromosome_14 |
| Location: | 3566424 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g631050 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTTGACTTTACGGCGGATGCAAGAGCGTC |
| Internal bar code: | GCCAGCCTAGATGGTAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 99.7513 |
| LEAP-Seq n confirming: | 6818 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCTACATAGCCTCTCAG |
| Suggested primer 2: | GCTGTTTCTCGTTTCAAGCC |