| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177303 |
| Chromosome: | chromosome 7 |
| Location: | 5338183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349900 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATCGGCCAAAATGGCCGATTAATGTCT |
| Internal bar code: | AGATAGGCGGGCTCAGTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 95.4309 |
| LEAP-Seq n confirming: | 8793 |
| LEAP-Seq n nonconfirming: | 421 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCACTCAGGTTTGTGGGT |
| Suggested primer 2: | ATGACCTTGTAGGCGTTTGG |