| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177310 |
| Chromosome: | chromosome 7 |
| Location: | 2722913 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331150 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATCGGCTCCTGTGACACGCACGCACGG |
| Internal bar code: | TAAGGCCTACGGCGTGAGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 4.58831 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 1518 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCTGCTCTGAGAACGAA |
| Suggested primer 2: | ATGTCCGACTACTGCAACCC |