Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.177325 |
Chromosome: | chromosome 7 |
Location: | 5478473 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g350850 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTACTGGAAAAGCCATGGGCATTTCATA |
Internal bar code: | GCGTCGAGTGAAGACCCTTCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 549 |
LEAP-Seq percent confirming: | 99.5602 |
LEAP-Seq n confirming: | 1132 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCCTGTAACTTGATGCAGA |
Suggested primer 2: | TATCGGCGTCATAATGCGTA |