| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177343 |
| Chromosome: | chromosome 14 |
| Location: | 809482 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613100 | (1 of 1) IPR008999 - Actin cross-linking | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCAGCACTTTGAATCGGAATTCAAAGG |
| Internal bar code: | GCGTGGCGATGTTATCCAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 98.983 |
| LEAP-Seq n confirming: | 292 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACATAAACAGCTTGGT |
| Suggested primer 2: | GTGCTTGCTTAACCTCTCCG |