Insertion junction: LMJ.RY0402.177344_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.177344
Chromosome:chromosome 13
Location:201657
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g563500 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGTCCCTGCGCCACGCCAGCTCCGTACG
Internal bar code:CTTTCCGGTGGAATGTGCGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:406
LEAP-Seq percent confirming:99.6947
LEAP-Seq n confirming:12410
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGGTCAACATCCCTCTCC
Suggested primer 2:ACCGAAACCACAGTCAGACC