Insertion junction: LMJ.RY0402.177344_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.177344
Chromosome:chromosome 13
Location:201658
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g563500 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTCATTGGACTGCCGGCCCGTCCGCGCG
Internal bar code:CCGCCCGAGACACCCGGCACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:680
LEAP-Seq percent confirming:58.5245
LEAP-Seq n confirming:944
LEAP-Seq n nonconfirming:669
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGGTCAACATCCCTCTCC
Suggested primer 2:ACCGAAACCACAGTCAGACC