| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177377 |
| Chromosome: | chromosome 16 |
| Location: | 6650343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671700 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAACATCGCTAGCTACCCCGCCCCCAT |
| Internal bar code: | CTCTTCGCTCCCCGCCAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 92.2812 |
| LEAP-Seq n confirming: | 2140 |
| LEAP-Seq n nonconfirming: | 179 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGATGTGAGGGCTGTCGT |
| Suggested primer 2: | AACAGCAGGTGGGTTGGTAG |