Insertion junction: LMJ.RY0402.177385_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.177385
Chromosome:chromosome 9
Location:6257199
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g406400 CTP3 (1 of 2) K17686 - Cu+-exporting ATPase (copA, ATP7); Copper transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCCAACTAACCCGTCTCCACGTGAACC
Internal bar code:GGGGAGTCTCGCGAAGCATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:616
LEAP-Seq percent confirming:99.895
LEAP-Seq n confirming:951
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCAATAAACACACGCAG
Suggested primer 2:CCCTGATCATCACGTTTGTG