Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.177429 |
Chromosome: | chromosome 3 |
Location: | 974620 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g148050 | POLI1 | DNA polymerase iota; (1 of 1) K03510 - DNA polymerase iota (POLI) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGCCTTGCATAGTCTGTATGCCCCACC |
Internal bar code: | CAGCAGCCGGGGGCGCAAAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 105 |
LEAP-Seq percent confirming: | 98.4456 |
LEAP-Seq n confirming: | 190 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCGGAAGTAAAACGCATAA |
Suggested primer 2: | ACAAACCAGACGACCAGACC |