| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.177435 |
| Chromosome: | chromosome 9 |
| Location: | 3871176 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392023 | CSV2 | Chlamydomonas-specific family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTTACTGTAATGCTCTGCTCAATAGTT |
| Internal bar code: | ACAAGCGGATGCTCTTTACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 99.4376 |
| LEAP-Seq n confirming: | 884 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGAGGTGAGAGGCGTGC |
| Suggested primer 2: | AAGTAACGGTGCTGATTGGG |