Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.177450 |
Chromosome: | chromosome 4 |
Location: | 1755413 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g212250 | (1 of 82) IPR020846 - Major facilitator superfamily domain; GT77 family protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTCCAGGCCGTACCTGCGTTCAGTAC |
Internal bar code: | GGCCCTGTGGCCGGGCCTTGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 131 |
LEAP-Seq percent confirming: | 97.2077 |
LEAP-Seq n confirming: | 557 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTTTGCTTCGTCGTCTCTC |
Suggested primer 2: | AGACGGTAGACCATCTTGCG |