| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177460 |
| Chromosome: | chromosome 16 |
| Location: | 7715532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCGCCCAGAACACTCGAGAGGCCTCT |
| Internal bar code: | AGAGGATCCGCGCTGTCGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1045 |
| LEAP-Seq percent confirming: | 93.3623 |
| LEAP-Seq n confirming: | 1505 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTAGTGAATGCCCCCTGG |
| Suggested primer 2: | GGCCACTGTTGAAGAGAAGG |