| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177464 |
| Chromosome: | chromosome_12 |
| Location: | 6486463 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g538450 | EPT1 | CDP-Ethanolamine:DAG Ethanolamine phosphotransferase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTGCGAGGATGCGGTATGCCCGGGCGTG |
| Internal bar code: | GCGTTTCAATGCACATGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 43.1461 |
| LEAP-Seq n confirming: | 960 |
| LEAP-Seq n nonconfirming: | 1265 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTCTGAGTTCGTGGCAG |
| Suggested primer 2: | CATAGGGCTTGCGTTTTCAT |