| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177466 |
| Chromosome: | chromosome 7 |
| Location: | 3322901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334700 | ATG101 | (1 of 1) PF07855 - Protein of unknown function (DUF1649) (DUF1649); Autophagy-related protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCGCCAACATCATGGGCGCATCACTGC |
| Internal bar code: | GAAGAGGGCCCCGCGGGACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 99.6307 |
| LEAP-Seq n confirming: | 1349 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGTGTGATTGGACACGC |
| Suggested primer 2: | TATACGACCACGGCCTTTTC |