| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177473 |
| Chromosome: | chromosome_3 |
| Location: | 717180 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g146067 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGTAGACAGCACACAGCAGGGCGGGTCCG |
| Internal bar code: | GGATGGTGTTTCTCGTATGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 99.5613 |
| LEAP-Seq n confirming: | 3631 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGATGTTGTTCTCGCTGA |
| Suggested primer 2: | GCATGCAGGTGATGATGTTC |