| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177485 |
| Chromosome: | chromosome 4 |
| Location: | 1247443 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215150 | SSS1B,SSS1,SSIb | (1 of 1) IPR023159 - SO1590-like domain; Soluble starch synthase IB | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCCCCTCACCTCCAGCAGAATGTCGGC |
| Internal bar code: | CTCTCGCGTAATAGACGCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 99.6633 |
| LEAP-Seq n confirming: | 296 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACTCCTACCCTCCTC |
| Suggested primer 2: | ATGCTCTGACACGCACTGAC |