| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177511 |
| Chromosome: | chromosome 2 |
| Location: | 954303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079850 | POLK1 | (1 of 1) K03511 - DNA polymerase kappa (POLK); DNA polymerase kappa | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTCATGATTCCCAGGTCCCCACAGCG |
| Internal bar code: | GCAACCTGTCTTCGTGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 971 |
| LEAP-Seq percent confirming: | 98.8304 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGTAACCGTCCTGGAA |
| Suggested primer 2: | TTTGCTCTGTGGTTTGCTTG |