| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177511 |
| Chromosome: | chromosome 2 |
| Location: | 5700422 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110050 | (1 of 1) PTHR22844:SF160 - WD40 REPEAT-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGATGTCGCCCGTGTTTGTGTCATACAG |
| Internal bar code: | TATTAACCCCCCCCCGATCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 99.9313 |
| LEAP-Seq n confirming: | 1454 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGACCTTGCTGCTCACCC |
| Suggested primer 2: | CCAGCAGACATGCAACAAGT |