| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.177513 |
| Chromosome: | chromosome 6 |
| Location: | 1289377 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258250 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTTGCCATACTTGCCAATAGCTCGCAA |
| Internal bar code: | TTGCGATTGGAGGGTCGCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1339 |
| LEAP-Seq percent confirming: | 99.5766 |
| LEAP-Seq n confirming: | 7291 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGGCGATGAGTGACTAC |
| Suggested primer 2: | CTCCAGCACAGACTGGATCA |