| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177516 |
| Chromosome: | chromosome 3 |
| Location: | 8015409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201500 | (1 of 1) PF16740 - Spindle and kinetochore-associated protein 2 (SKA2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTCGTGGATGGATGGGTGTTAGGCATC |
| Internal bar code: | GGCCTGTCGTGCGACTTGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 99.9514 |
| LEAP-Seq n confirming: | 2056 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAATGGGTTGGGGTTCAA |
| Suggested primer 2: | CCCAGAGGCTGAAGAAACTG |