| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177521 |
| Chromosome: | chromosome_14 |
| Location: | 413131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g610647 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTATTTCAAGGCCGCGTAATGTACTGAA |
| Internal bar code: | GCGAGACGGTGCACGCGAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 93.6881 |
| LEAP-Seq n confirming: | 1514 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCAGTAGAGGCTCCAAC |
| Suggested primer 2: | CTACTGCCTTTTCCGCTGAT |