| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.177523 |
| Chromosome: | chromosome 2 |
| Location: | 7952957 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141000 | DCL1 | Dicer-like protein; (1 of 1) K11592 - endoribonuclease Dicer (DICER1, DCR1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAATGCAGCAGGCAGAGGCAGCCGCAG |
| Internal bar code: | TTTGTGTGGTTGTGCCTGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.1158 |
| LEAP-Seq n confirming: | 1121 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGAGTGTAGGCATTTTGG |
| Suggested primer 2: | CTGGGTTTGAGGTGCATTTT |