| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177553 |
| Chromosome: | chromosome 12 |
| Location: | 1161011 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490050 | TPT18,GMT1,TPT19 | (1 of 3) K15356 - GDP-mannose transporter (VRG4, GONST1); Putative GDP-mannose transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGTAAAGGGAGTGGGCAGTATTGGATC |
| Internal bar code: | GAGGGGGTCGATCCACGGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 95.5157 |
| LEAP-Seq n confirming: | 426 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTAAGCCGTTATTCGCC |
| Suggested primer 2: | TTCGACTGATCATTTGCTGC |