| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177600 |
| Chromosome: | chromosome 2 |
| Location: | 5659768 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109650 | GSP1 | Gamete-specific plus 1; (1 of 2) IPR001356//IPR008422//IPR009057 - Homeobox domain // Homeobox KN domain // Homeodomain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTCTGGGCCGCGTCTAAGGCAGGGTAT |
| Internal bar code: | AGGACCGAAGCATTGACGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 97.4955 |
| LEAP-Seq n confirming: | 545 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACTTCTGGGCCTGTCTG |
| Suggested primer 2: | CCGAGCAGTAACAGCATGAA |