| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177609 |
| Chromosome: | chromosome 7 |
| Location: | 1441438 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323150 | TEH4 | (1 of 1) K17362 - acyl-coenzyme A thioesterase 13 [EC:3.1.2.-] (ACOT13); Thioesterase-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTACCGTACTGTAACCGGCAATTGCAA |
| Internal bar code: | TGCGGACTCACTTGGTGTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 98.3753 |
| LEAP-Seq n confirming: | 1211 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGGCTTGAGACTTGAGA |
| Suggested primer 2: | CACGACTACCCACTCCCACT |