| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177612 |
| Chromosome: | chromosome 8 |
| Location: | 4445220 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382250 | (1 of 4) PTHR11584//PTHR11584:SF377 - SERINE/THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAGGTTTCCGCCATCGCTGCTCGAGGA |
| Internal bar code: | CGTACCCCTCAGTATAGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.8789 |
| LEAP-Seq n confirming: | 2474 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTCGCAAATGGAGTAT |
| Suggested primer 2: | GTGCCATAGAGAAAGGCAGC |