| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177623 |
| Chromosome: | chromosome 7 |
| Location: | 2131157 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCAGCGGTGCTCGGTCTGCATGACACT |
| Internal bar code: | GAGGCAGCCTCTGTGTATCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 955 |
| LEAP-Seq percent confirming: | 98.7925 |
| LEAP-Seq n confirming: | 2209 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGACGGCTTCACGAGTA |
| Suggested primer 2: | GGCAGGTTGTCGTGGAGTAT |