| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177631 |
| Chromosome: | chromosome 9 |
| Location: | 709570 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402700 | (1 of 3) K12041 - solute carrier family 9 (sodium/hydrogen exchanger), member 6/7 (SLC9A6_7, NHE6_7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGGACCCACTGCGTTGGGCGGTGAGTG |
| Internal bar code: | GAGCGCGGGCTGAGAAAAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 99.4116 |
| LEAP-Seq n confirming: | 6420 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGGTTGTGCAGCATGG |
| Suggested primer 2: | ACCTTCCGTACTGTGCCATC |