| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177632 |
| Chromosome: | chromosome 2 |
| Location: | 1036426 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080600 | BIP2 | (1 of 2) K09490 - heat shock 70kDa protein 5 (HSPA5, BIP); Endoplasmic reticulum associated Hsp70 protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGGGCCGGGGAGGTGCGTGCCTGCGT |
| Internal bar code: | GCGATCGTGGTGGTGGGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1138 |
| LEAP-Seq percent confirming: | 99.351 |
| LEAP-Seq n confirming: | 1990 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCCTCACCTTACTCC |
| Suggested primer 2: | AGTCACCAACAACCCTCCAG |