Insertion junction: LMJ.RY0402.177647_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.177647
Chromosome:chromosome 2
Location:5106822
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g104951 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTAGCCTGCGCCGCTGCAGCTTCTTGT
Internal bar code:GAGCTAAGCGTGGCTCTCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:362
LEAP-Seq percent confirming:95.53
LEAP-Seq n confirming:748
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCGAACCTCCTCTAGCTCCT
Suggested primer 2:AAGGTCCAGGGCAGTGTATG