| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177651 |
| Chromosome: | chromosome 7 |
| Location: | 2317258 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGATACAGTATCTATCGGATGGGGCAT |
| Internal bar code: | TGTGAACTTATCTTACCAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 83.7144 |
| LEAP-Seq n confirming: | 1794 |
| LEAP-Seq n nonconfirming: | 349 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGGATTGGAAAGTGGA |
| Suggested primer 2: | GGACTTGTTCACTGGGTGGT |