| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177659 |
| Chromosome: | chromosome 2 |
| Location: | 3962648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGCGGTGTGCGGTGCTGCACTACCGT |
| Internal bar code: | GACGGTATAGTGTCCAAAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 96.6234 |
| LEAP-Seq n confirming: | 6324 |
| LEAP-Seq n nonconfirming: | 221 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCGCACGTGATGATATG |
| Suggested primer 2: | CATCTAACAACGGTGGGCTT |