| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177664 |
| Chromosome: | chromosome 7 |
| Location: | 2848918 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332100 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGCGGCCCTTACTCAGGACCTCGGGG |
| Internal bar code: | CCGCAGAGTCGAGCCGGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 99.6374 |
| LEAP-Seq n confirming: | 1099 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCGTCATTGGTGGTGTA |
| Suggested primer 2: | GGTCTCGGTGAGTGGATGAT |