| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177700 |
| Chromosome: | chromosome 7 |
| Location: | 3612630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGCATGCAGTGTGTGTTTTGACGTAGC |
| Internal bar code: | GGCGCAAAAGATTTGAAGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 99.8487 |
| LEAP-Seq n confirming: | 660 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACCTAACGCCCAACACC |
| Suggested primer 2: | TTCGGTTGCAATAGAGGGAC |