Insertion junction: LMJ.RY0402.177711_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.177711
Chromosome:chromosome 2
Location:2369716
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g091000 (1 of 1) PF05346 - Eukaryotic membrane protein family (DUF747) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCACGGCTGCTGCAGTCCGGCGGCTGG
Internal bar code:GCGTGTAAAGCATTACCTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:567
LEAP-Seq percent confirming:99.8043
LEAP-Seq n confirming:510
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCAATCCTTCATCCCTGA
Suggested primer 2:TTGGTAGGTAGGTTGGCTGG