| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.177714 |
| Chromosome: | chromosome_12 |
| Location: | 3344296 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g497450 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGGGTCCTCGCTCGCCTCCAGGCCCCGCA |
| Internal bar code: | CCAAAGGACGTGGTGGAGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 1.93548 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGGAATGCAGGTCTAAG |
| Suggested primer 2: | GCAACGCAGCTGTACGATTA |