| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177745 |
| Chromosome: | chromosome 17 |
| Location: | 4238235 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730483 | (1 of 1) IPR000104//IPR016181 - Antifreeze protein, type I // Acyl-CoA N-acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCTCTCACCTGACCCAAACAACGCAGC |
| Internal bar code: | GTGGCCACGACTGTAAGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 99.3092 |
| LEAP-Seq n confirming: | 1150 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCCTGCAGTTCTCCTTC |
| Suggested primer 2: | CCTCTCAGTCCAGAACCTGC |