Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.177794 |
Chromosome: | chromosome 8 |
Location: | 3364015 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g374200 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTTACTCGCCTCGCGCGACGCAGCCTC |
Internal bar code: | GTGGGGTTGAGGGAAGGGGCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 426 |
LEAP-Seq percent confirming: | 98.3139 |
LEAP-Seq n confirming: | 1516 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCGGAGCTCAAAAGTACAG |
Suggested primer 2: | GTCCACTTGCCAAACCTTGT |