| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.177798 |
| Chromosome: | chromosome 6 |
| Location: | 6565672 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293516 | DEGO2 | (1 of 4) PTHR22939:SF83 - PROTEASE DO-LIKE 2, CHLOROPLASTIC; Inactive Deg protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCTCAACACAAGCTTGTGATGCCCTA |
| Internal bar code: | CTTGGCGGCCTGCCGGAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 95.7395 |
| LEAP-Seq n confirming: | 3573 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGATGATGATGAGGCG |
| Suggested primer 2: | GTGAACTTCTGGAACGCCAT |