| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177858 |
| Chromosome: | chromosome 12 |
| Location: | 5904023 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534050 | FAP366 | (1 of 1) PTHR10728 - CYTOSOLIC PHOSPHOLIPASE A2; Flagellar Associated Protein 366 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGACGAGGCCAGCCAGTGGCGTTTGCT |
| Internal bar code: | GCGGGTCTTACCGGGCTCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 99.8028 |
| LEAP-Seq n confirming: | 2024 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTGTGGAGAGAAAGAG |
| Suggested primer 2: | ACTTCCAGCAGAGGACCTGA |