| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.177870 |
| Chromosome: | chromosome 9 |
| Location: | 2373772 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391900 | TRXH1,TRXH,TRXh1,Chh1,TRX5 | (1 of 9) K03671 - thioredoxin 1 (trxA); Thioredoxin h1, cytosolic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAACCCTTTCCTCGCTTCCTTCCCGCAG |
| Internal bar code: | CACCTGTCTCAAGACCAGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 946 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCTGGCAGATTGTTGTC |
| Suggested primer 2: | GAGTGTCTCACTCAACGCCA |