| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.177899 |
| Chromosome: | chromosome 3 |
| Location: | 3730211 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169650 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTCTTGACCACGAGGGTCTTCGCCGC |
| Internal bar code: | CCGTCGGAACGGACATACGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.7238 |
| LEAP-Seq n confirming: | 722 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTTCGTAAGCTTCGTCT |
| Suggested primer 2: | GTCGCTTCCTCAACGCTATC |